A radical switch in clonality reveals a stem cell niche in the epiphyseal growth plate

Nature. 2019 Mar;567(7747):234-238. doi: 10.1038/s41586-019-0989-6. Epub 2019 Feb 27.


Longitudinal bone growth in children is sustained by growth plates, narrow discs of cartilage that provide a continuous supply of chondrocytes for endochondral ossification1. However, it remains unknown how this supply is maintained throughout childhood growth. Chondroprogenitors in the resting zone are thought to be gradually consumed as they supply cells for longitudinal growth1,2, but this model has never been proved. Here, using clonal genetic tracing with multicolour reporters and functional perturbations, we demonstrate that longitudinal growth during the fetal and neonatal periods involves depletion of chondroprogenitors, whereas later in life, coinciding with the formation of the secondary ossification centre, chondroprogenitors acquire the capacity for self-renewal, resulting in the formation of large, stable monoclonal columns of chondrocytes. Simultaneously, chondroprogenitors begin to express stem cell markers and undergo symmetric cell division. Regulation of the pool of self-renewing progenitors involves the hedgehog and mammalian target of rapamycin complex 1 (mTORC1) signalling pathways. Our findings indicate that a stem cell niche develops postnatally in the epiphyseal growth plate, which provides a continuous supply of chondrocytes over a prolonged period.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging
  • Animals
  • Cartilage / cytology
  • Cell Self Renewal
  • Chondrocytes / cytology*
  • Clone Cells / cytology*
  • Clone Cells / metabolism
  • Female
  • Growth Plate / cytology*
  • Growth Plate / metabolism
  • Male
  • Mechanistic Target of Rapamycin Complex 1 / metabolism
  • Mice
  • Stem Cell Niche / physiology*


  • Mechanistic Target of Rapamycin Complex 1