Biofilm is a serious issue in the dairy factory due to it increases the opportunity for microbial contamination. Staphylococcus aureus and Pseudomonas aeruginosa are the bacteria capable to construct the biofilm on materials and equipments. Therefore, the bacterial growth and efficiency of sanitizing agents to solve the problems were evaluated. These bacteria grew well in UHT milk when they were cultivated at 37 °C, especially S. aureus. The exponential growth phase and biofilm on stainless steel were discovered by short contact time at 2 h. The mature stage of biofilm cycle was found at 4 h during bacteria growth and it was continuously constructed until 48 h. The 10, 24, and 48 h-old biofilm adhering on stainless steel were established with oxisan and chlorine used as microbial sanitizers. The 4% of sanitizing agents was the efficiency concentration to reduce biofilm on stainless steel up to 82% when these bacteria grew in UHT milk.
Keywords: Biofilm; Chlorine; Oxisan; Pseudomonas aeruginosa; Staphylococcus aureus.