Dietary selenomethionine increases antioxidant capacity of geese by improving glutathione and thioredoxin systems

Poult Sci. 2019 Sep 1;98(9):3763-3769. doi: 10.3382/ps/pez066.

Abstract

A total of 200 healthy 28-day-old male Jiangnan White geese were used to investigate the free radical scavenging ability, reduced glutathione (GSH) and thioredoxin systems, and the concentrations of reactive oxygen metabolites (ROM), malondialdehyde (MDA), and protein carbonyl (PC) in geese fed diets with organic selenium (Se) (Selenomethionine, SeMet) and inorganic Se (sodium selenite, SS). All geese were randomly allotted into 4 groups with 5 replicates of 10 geese each, and received basal diet supplemented with 0.3 mg Se/kg SS, 0.2, 0.3 and 0.4 mg Se/kg SeMet until 70 D of age, respectively. Geese in the SS and SeMet groups exhibited similar growth performance. Diet with SeMet increased the scavenging abilities of 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical (ABTS•+, P < 0.001) and superoxide radical (O2-•, P = 0.002) in the serum of geese, as well as the scavenging abilities of ABTS•+ (P = 0.023), hydroxyl radical (P = 0.009) and O2-• (P = 0.019) in the liver of geese. Compared to the SS group, SeMet increased hepatic GSH concentration (P = 0.002), the activities of glutathione peroxidase (P = 0.031), γ-glutamate cysteine ligase (P < 0.001), and thioredoxin reductase (P < 0.001), and decreased the concentrations of ROM, MDA, and PC in the serum and liver of geese (P < 0.05). In conclusion, dietary SeMet inclusion would be more effective than SS in increasing the antioxidant capacity of geese, possibly by improving GSH and thioredoxin systems, and 0.2 mg Se/kg SeMet in goose diet is recommended.

Keywords: GSH; antioxidant capacity; goose; selenomethionine; thioredoxin.

Publication types

  • Comparative Study

MeSH terms

  • Animal Feed / analysis
  • Animals
  • Antioxidants / metabolism*
  • Avian Proteins / metabolism
  • Diet / veterinary
  • Dietary Supplements / analysis
  • Geese / metabolism*
  • Glutathione / metabolism*
  • Male
  • Random Allocation
  • Selenomethionine / administration & dosage
  • Selenomethionine / metabolism*
  • Sodium Selenite / administration & dosage
  • Sodium Selenite / metabolism*
  • Thioredoxins / metabolism*

Substances

  • Antioxidants
  • Avian Proteins
  • Thioredoxins
  • Selenomethionine
  • Glutathione
  • Sodium Selenite