Adrenic acid non-enzymatic peroxidation products in biofluids of moderate preterm infants

Free Radic Biol Med. 2019 Oct:142:107-112. doi: 10.1016/j.freeradbiomed.2019.02.024. Epub 2019 Feb 25.

Abstract

Oxidative stress plays an essential role in processes of signaling and damage to biomolecules during early perinatal life. Isoprostanoids and isofuranoids from the free radical-catalyzed peroxidation of polyunsaturated fatty acids (PUFAs) are widely recognized as reliable biomarkers of oxidative stress. However, their quantification is not straightforward due to high structural similarity of the compounds formed. In this work, a semiquantitative method for the analysis of adrenic acid (AdA, C22:4 n-6) non-enzymatic peroxidation products (i.e. dihomo-isoprostanes and dihomo-isofurans) was developed. The proposed ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) method was applied to the analysis of blood plasma and urine from preterm infants providing information about AdA peroxidation.

Keywords: Dihomo-isofurans; Dihomo-isoprostanes; Lipid peroxidation; Preterm infants; UPLC-MS/MS.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / standards
  • Fatty Acids, Unsaturated / blood
  • Fatty Acids, Unsaturated / urine*
  • Furans / blood
  • Furans / urine*
  • Humans
  • Infant, Newborn
  • Infant, Premature
  • Isoprostanes / blood
  • Isoprostanes / urine*
  • Lipid Peroxidation / physiology
  • Oxidative Stress
  • Reactive Oxygen Species / blood
  • Reactive Oxygen Species / urine*
  • Tandem Mass Spectrometry / standards

Substances

  • Fatty Acids, Unsaturated
  • Furans
  • Isoprostanes
  • Reactive Oxygen Species
  • adrenic acid