Draft Genome of Burkholderia cenocepacia TAtl-371, a Strain from the Burkholderia cepacia Complex Retains Antagonism in Different Carbon and Nitrogen Sources

Curr Microbiol. 2019 May;76(5):566-574. doi: 10.1007/s00284-019-01657-0. Epub 2019 Feb 28.

Abstract

Burkholderia cenocepacia TAtl-371 was isolated from the rhizosphere of a tomato plant growing in Atlatlahucan, Morelos, Mexico. This strain exhibited a broad antimicrobial spectrum against bacteria, yeast, and fungi. Here, we report and describe the improved, high-quality permanent draft genome of B. cenocepacia TAtl-371, which was sequenced using a combination of PacBio RS and PacBio RS II sequencing methods. The 7,496,106 bp genome of the TAtl-371 strain is arranged in three scaffolds, contains 6722 protein-coding genes, and 99 RNA only-encoding genes. Genome analysis revealed genes related to biosynthesis of antimicrobials such as non-ribosomal peptides, siderophores, chitinases, and bacteriocins. Moreover, analysis of bacterial growth on different carbon and nitrogen sources shows that the strain retains its antimicrobial ability.

MeSH terms

  • Antibiosis*
  • Bacteriocins / genetics
  • Burkholderia cenocepacia / genetics*
  • Burkholderia cenocepacia / isolation & purification
  • Burkholderia cepacia complex*
  • Carbon / metabolism*
  • Chitinases / genetics
  • Genome, Bacterial*
  • Lycopersicon esculentum / microbiology
  • Mexico
  • Nitrogen / metabolism*
  • Rhizosphere
  • Sequence Analysis, DNA
  • Siderophores / genetics
  • Soil Microbiology

Substances

  • Bacteriocins
  • Siderophores
  • Carbon
  • Chitinases
  • Nitrogen