ZOO: an automatic data-collection system for high-throughput structure analysis in protein microcrystallography

Acta Crystallogr D Struct Biol. 2019 Feb 1;75(Pt 2):138-150. doi: 10.1107/S2059798318017795. Epub 2019 Jan 28.


Owing to the development of brilliant microfocus beamlines, rapid-readout detectors and sample changers, protein microcrystallography is rapidly becoming a popular technique for accessing structural information from complex biological samples. However, the method is time-consuming and labor-intensive and requires technical expertise to obtain high-resolution protein crystal structures. At SPring-8, an automated data-collection system named ZOO has been developed. This system enables faster data collection, facilitates advanced data-collection and data-processing techniques, and permits the collection of higher quality data. In this paper, the key features of the functionality put in place on the SPring-8 microbeam beamline BL32XU are described and the major advantages of this system are outlined. The ZOO system will be a major driving force in the evolution of the macromolecular crystallography beamlines at SPring-8.

Keywords: ZOO; automated data collection; protein microcrystallography.

MeSH terms

  • Animals
  • Crystallography, X-Ray / economics
  • Crystallography, X-Ray / instrumentation
  • Crystallography, X-Ray / methods*
  • Data Collection / economics
  • Data Collection / instrumentation
  • Data Collection / methods*
  • Humans
  • Muramidase / chemistry
  • Protein Conformation
  • Proteins / chemistry*
  • Receptor, Muscarinic M2 / chemistry
  • Software*
  • Thermolysin / chemistry


  • Proteins
  • Receptor, Muscarinic M2
  • Muramidase
  • Thermolysin