Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members

Neuron. 2019 Apr 3;102(1):159-172.e7. doi: 10.1016/j.neuron.2019.01.051. Epub 2019 Feb 26.


Adult neural stem cells and multiciliated ependymal cells are glial cells essential for neurological functions. Together, they make up the adult neurogenic niche. Using both high-throughput clonal analysis and single-cell resolution of progenitor division patterns and fate, we show that these two components of the neurogenic niche are lineally related: adult neural stem cells are sister cells to ependymal cells, whereas most ependymal cells arise from the terminal symmetric divisions of the lineage. Unexpectedly, we found that the antagonist regulators of DNA replication, GemC1 and Geminin, can tune the proportion of neural stem cells and ependymal cells. Our findings reveal the controlled dynamic of the neurogenic niche ontogeny and identify the Geminin family members as key regulators of the initial pool of adult neural stem cells.

Keywords: GemC1; cell division; cell lineage; geminin family; multiciliated ependymal cells; multiciliogenesis; neural stem cells; radial glial cells; symmetric division.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Adult Stem Cells / cytology
  • Adult Stem Cells / metabolism
  • Animals
  • Astrocytes / cytology*
  • Astrocytes / metabolism
  • Carrier Proteins / metabolism
  • Cell Cycle Proteins
  • Cell Lineage
  • DNA Replication
  • Electroporation
  • Embryo, Mammalian
  • Ependyma / cytology*
  • Ependymoglial Cells / cytology*
  • Ependymoglial Cells / metabolism
  • Geminin / metabolism
  • Mice
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / metabolism


  • Carrier Proteins
  • Cell Cycle Proteins
  • Geminin
  • Gmnc protein, mouse
  • Gmnn protein, mouse