Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities

Proc Natl Acad Sci U S A. 1986 Apr;83(7):2061-5. doi: 10.1073/pnas.83.7.2061.

Abstract

A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from a mouse helper T-cell cDNA library. The library, constructed in the pcD expression vector, was screened by transfecting COS monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the transfected cell supernatants, we identified clones encoding a factor that stimulates T-cell and mast cell lines. This factor also induces Ia expression on resting B cells and enhances IgG1 and IgE production by B cells, two properties of B-cell-stimulatory factor 1. The DNA sequence codes for a polypeptide of 140 amino acid residues including a putative signal peptide. These results demonstrate that a single cDNA clone distinct from interleukin 2 and interleukin 3 encodes a polypeptide with multiple biological activities.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / cytology*
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Growth Substances / genetics*
  • Growth Substances / physiology
  • Interleukin-2 / genetics*
  • Interleukin-2 / physiology
  • Interleukin-3
  • Interleukin-4
  • Lymphokines / genetics*
  • Lymphokines / physiology
  • Mast Cells / cytology*
  • Mice
  • T-Lymphocytes / cytology
  • T-Lymphocytes / physiology*

Substances

  • Growth Substances
  • Interleukin-2
  • Interleukin-3
  • Lymphokines
  • Interleukin-4
  • DNA

Associated data

  • GENBANK/M13238