Topical application and oral supplementation of peptides in the improvement of skin viscoelasticity and density

J Cosmet Dermatol. 2019 Dec;18(6):1693-1699. doi: 10.1111/jocd.12893. Epub 2019 Mar 4.


Background: The combination of oral supplementation and topical formulations to the improvement of skin conditions has been proposed as an innovative approach to obtain effective treatments. However, studies comparing the effectiveness of each type of treatments are still in lack. This way, the objective of this work was to evaluate the clinical efficacy of a dermocosmetic formulation with di- and tripeptides, as well the effects of an oral supplementation based on hydrolyzed collagen through biophysical and skin imaging techniques.

Methods: Sixty healthy female subjects, aged between 40 and 50 years, were enrolled, being separated in 3 groups: topical formulation, oral supplementation, and oral placebo. The stratum corneum water content, skin viscoelasticity, dermis echogenicity, and skin pores parameters were evaluated.

Results: The group with the topical formulation showed a significant increase in the stratum corneum water content and skin elasticity after 28-day period and also acted in the dermis echogenicity after 90 days with the formulation with peptides. The oral supplementation acted on skin elasticity and presented a more pronounced effect on dermis echogenicity, reducing skin pores after 90-day period.

Conclusion: The obtained results with oral supplementation and topical application of hydrolyzed proteins were considered complementary in the improvement of general skin conditions, acting in different mechanisms.

Keywords: biophysical and skin imaging techniques; dermocosmetic formulation; hydrolyzed collagen; oral supplementation; rice peptides.

Publication types

  • Comparative Study
  • Randomized Controlled Trial

MeSH terms

  • Administration, Cutaneous
  • Administration, Oral
  • Adult
  • Dietary Supplements*
  • Elasticity / drug effects
  • Female
  • Healthy Volunteers
  • Humans
  • Middle Aged
  • Peptides / administration & dosage*
  • Photography
  • Skin / diagnostic imaging
  • Skin / drug effects*
  • Skin / metabolism
  • Skin Aging / drug effects*
  • Skin Care / methods*
  • Ultrasonography
  • Viscosity / drug effects


  • Peptides