Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2019 Jun;38(6):1113-1122.
doi: 10.1007/s10096-019-03506-1. Epub 2019 Mar 5.

Role of Mycobacterium Avium Lysate INF-γ, IL-17, and IL-2 ELISPOT Assays in Diagnosing Nontuberculous Mycobacteria Lymphadenitis in Children

Affiliations
Comparative Study

Role of Mycobacterium Avium Lysate INF-γ, IL-17, and IL-2 ELISPOT Assays in Diagnosing Nontuberculous Mycobacteria Lymphadenitis in Children

Chiara Della Bella et al. Eur J Clin Microbiol Infect Dis. .

Abstract

Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.

Keywords: Children; ELISPOT; Lymphadenitis; Mycobacterium avium; Nontuberculous mycobacteria.

Similar articles

See all similar articles

References

    1. J Infect Dis. 2001 Dec 1;184(11):1480-4 - PubMed
    1. Arch Otolaryngol Head Neck Surg. 2003 Mar;129(3):341-4 - PubMed
    1. J Clin Microbiol. 2004 Jul;42(7):3000-11 - PubMed
    1. Clin Infect Dis. 2004 Aug 15;39(4):450-6 - PubMed
    1. J Otolaryngol. 2005 Jun;34 Suppl 1:S40-4 - PubMed

Publication types

MeSH terms

LinkOut - more resources

Feedback