Organ cultures of human fetal hepatocytes in the study of extra-and intracellular alpha1-antitrypsin

Biochim Biophys Acta. 1978 Sep 6;542(3):496-505. doi: 10.1016/0304-4165(78)90379-3.


The rate of synthesis of alpha 1-antitrypsin has been studied in organ cultures of fetal human liver. By de novo synthesis, alpha 1-antitrypsin of the same electrophoretic mobility and molecular size as plasma alpha 1-antitrypsin was produced. Synthetic rate was comparable to in vivo conditions and was suppressed by cycloheximide, colchicine and neuraminidase. By increasing alpha 1-antitrypsin levels in cultre medium, suppression of alpha 1-antitrypsin release from the intra-to the extracellular site was achieved, i.e., synthesis does not proceed autonomously. This suppression was preceded by a temporary enhancement of synthesis. Both effects were found to be independent of degree of sialylation of add-d alpha 1-antitrypsin. In contrast to alpha 1-antitrypsin released in tissue culture, the intracellular protein, as analyzed by crossed immunoelectrophoresis of Triton X-100 extracts from fetal liver, was found to occur partly as slowly moving peaks. Whether these peaks represent proforms or incompletely glycosylated precursors of export alpha 1-antitrypsin or complexes with proteases remains unsettled. A variety of other plasma proteins are released in organ cultures making the system suitable for study of factors regulating plasma protein synthesis.

MeSH terms

  • Culture Media
  • Extracellular Space / metabolism
  • Glycoproteins / metabolism
  • Humans
  • Liver / embryology
  • Liver / metabolism*
  • Neuraminidase / metabolism
  • Organ Culture Techniques
  • alpha 1-Antitrypsin / biosynthesis
  • alpha 1-Antitrypsin / metabolism*


  • Culture Media
  • Glycoproteins
  • alpha 1-Antitrypsin
  • Neuraminidase