Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-Mesenchymal Interactions

Methods Mol Biol. 2019;1922:49-55. doi: 10.1007/978-1-4939-9012-2_6.

Abstract

Tissue interactions are crucial during the development of organs. Among the most studied tissue interactions are those that take place between the epithelial cells and the underlying mesenchymal cells, known as epithelial-mesenchymal interactions. Tissue recombination assay is a valuable model to study the mechanisms involved in the regulation of such interactions. Here, we describe how to dissociate and recombine the epithelial and mesenchymal components of the embryonic tooth. In addition, we explain how to transplant the recombined tissues under the kidney capsule of immunocompromised mice in order to allow their further development into a mature tooth.

Keywords: Embryo; Epithelial-mesenchymal interactions; Kidney capsule transplantation; Organogenesis; Tissue recombination; Tooth.

MeSH terms

  • Animals
  • Embryo, Mammalian
  • Embryonic Development*
  • Epithelial Cells / physiology*
  • Mesenchymal Stem Cells / physiology*
  • Mice
  • Subrenal Capsule Assay / methods*
  • Tooth / cytology*
  • Tooth / embryology