In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nanofilms

Yi Zou; Lei Wan; Jenifer Blacklock; Lingxiao Xie; Sean Carroll; David Oupicky; Guangzhao Mao

doi:10.1007/978-1-4939-9092-4_13

In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nanofilms

Methods Mol Biol. 2019:1943:199-209. doi: 10.1007/978-1-4939-9092-4_13.

Authors

Yi Zou¹, Lei Wan¹, Jenifer Blacklock², Lingxiao Xie¹, Sean Carroll^{1

2}, David Oupicky^{3

4}, Guangzhao Mao⁵

Affiliations

¹ Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, MI, USA.
² Department of Biomedical Engineering, Wayne State University, Detroit, MI, USA.
³ Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI, USA.
⁴ University of Nebraska Medical Center, Omaha, NE, USA.
⁵ Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, MI, USA. guangzhao.mao@wayne.edu.

PMID: 30838618
DOI: 10.1007/978-1-4939-9092-4_13

Abstract

Synthetic vector-based gene delivery continues to gain strength as viable alternatives to viral vectors due to safety and other concerns. DNA release dynamics is key to the understanding and control of gene delivery from nanosystems. Here we describe atomic force microscope (AFM) application to the understanding of DNA release dynamics from bioreducible polycation-based nanosystems. The two nanosystems are polyplex nanoparticles and layer-by-layer (LbL) films.

Keywords: DNA release; Gene delivery; In situ AFM; Layer-by-layer (LbL) film; Polyplex.

MeSH terms

DNA / chemistry*
DNA / ultrastructure
Microscopy, Atomic Force / methods*
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Polyamines / chemistry*
Polyelectrolytes
Time-Lapse Imaging / methods

Substances

Polyamines
Polyelectrolytes
polycations
DNA