Dosage requirements for runt in the segmentation of Drosophila embryos

Cell. 1986 Apr 25;45(2):289-99. doi: 10.1016/0092-8674(86)90393-4.


The runt gene is required in a Drosophila embryo for normal segmentation. We investigate this requirement by analyzing runt mutations of varying strength and by manipulating wild-type gene dosage. Elimination of runt causes periodic deletions in the segmentation pattern which are spaced at two segment intervals along the antero-posterior axis. The pattern deletions produced by partial loss of function mutations and by halving the normal wild-type gene dosage reveal a gradation in the requirement for runt, with the centers of the affected regions being most sensitive to deletion. Significantly, increased runt+ dosage causes an anti-runt phenotype consisting of periodic pattern deletions that are out of phase with those caused by runt mutations.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Embryo, Nonmammalian / physiology
  • Embryonic and Fetal Development
  • Epistasis, Genetic
  • Female
  • Genes*
  • Larva / ultrastructure
  • Male
  • Mutation
  • Phenotype