Temporal-controlled bioactive molecules releasing core-shell nano-system for tissue engineering strategies in endodontics

Nanomedicine. 2019 Jun:18:11-20. doi: 10.1016/j.nano.2019.02.013. Epub 2019 Mar 4.

Abstract

Temporal-controlled release of bioactive molecules is of key importance in the clinical translation of tissue engineering techniques. We engineered a core-shell nano-system (TD-NS) that sequentially released transforming growth factor-β1 (TGF-β1), a chemotactic/proliferating growth factor and dexamethasone (Dex), an osteo/odontogenic agent in a temporal-controlled manner. In stage-1, there was a rapid release of TGF-β1, reaching a concentration of 2 ng/mL of TGF-β1 in 7 days to 14 days, which tapers subsequently. In stage-2, Dex was released linearly from 9 days to 28 days. The TD-NS group showed a significantly higher (P < 0.05) osteo/odontogenic differentiation compared to the control and free TGF-β1 group (Free-TD) that was further corroborated with animal models/histochemical examination. The findings from this study highlighted the potential of temporal-controlled delivery of TGF-β1 and Dex from a single nano-carrier to direct spatial and temporal-control for a cell-free tissue engineering strategy in the treatment of apical periodontitis.

Keywords: Core-shell nano-system; Dexamethasone; Odontogenic differentiation; Stem cells from apical papilla; TGF-β1; Temporal-controlled release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Biocompatible Materials / pharmacology*
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chemotaxis / drug effects
  • Dental Papilla / cytology
  • Dexamethasone / pharmacology
  • Endodontics / methods*
  • Guinea Pigs
  • Humans
  • Male
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Odontogenesis / drug effects
  • Stem Cells / cytology
  • Stem Cells / drug effects
  • Stem Cells / enzymology
  • Time Factors
  • Tissue Engineering / methods*
  • Transforming Growth Factor beta1 / pharmacology

Substances

  • Biocompatible Materials
  • Transforming Growth Factor beta1
  • Dexamethasone
  • Alkaline Phosphatase