Microtubule-associated protein tau. A component of Alzheimer paired helical filaments

J Biol Chem. 1986 May 5;261(13):6084-9.

Abstract

Microtubule-associated protein tau was purified from bovine brain microtubules by either (1) phosphocellulose chromatography, (2) heat treatment at pH 6.4, (3) heat treatment at pH 2.7, (4) heat treatment at pH 2.7 followed by extraction with perchloric acid and precipitation with glycerol, or (5) by precipitation with ammonium sulfate followed by extraction with perchloric acid. All of these tau preparations reacted specifically with antibodies to Alzheimer paired helical filaments. Affinity purified antibodies to tau labeled both Alzheimer neurofibrillary tangles and plaque neurites but not amyloid in Alzheimer brain tissue sections and labeled paired helical filament polypeptides on Western blots. Human brain tau and paired helical filament polypeptides co-migrated on sodium dodecyl sulfate-polyacrylamide gels. These results suggest that tau is a major component of Alzheimer paired helical filaments.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alzheimer Disease / metabolism*
  • Animals
  • Antibodies
  • Antigen-Antibody Complex
  • Brain Chemistry*
  • Cattle
  • Chromatography, Affinity
  • Cross Reactions
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Microtubule-Associated Proteins / isolation & purification*
  • Microtubules / ultrastructure*
  • Molecular Weight
  • Nerve Tissue Proteins / isolation & purification*
  • Species Specificity
  • tau Proteins

Substances

  • Antibodies
  • Antigen-Antibody Complex
  • Microtubule-Associated Proteins
  • Nerve Tissue Proteins
  • tau Proteins