Coriandrum sativum seeds extract mitigate progression of diabetic nephropathy in experimental rats via AGEs inhibition

PLoS One. 2019 Mar 7;14(3):e0213147. doi: 10.1371/journal.pone.0213147. eCollection 2019.

Abstract

Inthe present study, we have demonstrated the phytochemical composition of petroleum ether extract of C. sativum (CPE) seeds by using chromatographic, spectroscopic as well spectrometric analysis. CPE was evaluated for its possible role in mitigation of diabetic nephropathy (DN) in Streptozotocin (STZ)-nicotinamide (NAD) induced type 2 diabetes model. Administration of CPE at doses of 100, 200, and 400 mg/kg for 45 days has produced significant attenuation of elevated biochemical parameters including serum glucose, lipid and creatinine levels. CPE has also reserved albuminuria and elevated creatinine clearance in treated diabetic rats. Advanced glycation end products (AGEs) formation in kidneyswas also considerably reduced along with noteworthy increase in level of superoxide dismutase (SOD), glutathione (GSH), and decrease in lipid peroxidation in terms of thiobarbituric acid reactive species (TBARS). Molecular docking studies were also employed to reveal out the possible mechanism. In conclusion, using STZ-NAD model, we have successfully predicted that by assets of bioactive constituents CPE might inhibit the progression of DN. C. sativum may act as potential adjuvant for antidiabetic therapy and needs to be investigated further.

MeSH terms

  • Animals
  • Binding Sites
  • Blood Glucose / analysis
  • Coriandrum / chemistry*
  • Coriandrum / metabolism
  • Creatinine / blood
  • Diabetes Mellitus, Experimental / chemically induced
  • Diabetes Mellitus, Experimental / complications
  • Diabetic Nephropathies / drug therapy
  • Diabetic Nephropathies / pathology*
  • Gas Chromatography-Mass Spectrometry
  • Glutathione / metabolism
  • Glycation End Products, Advanced / antagonists & inhibitors
  • Glycation End Products, Advanced / metabolism*
  • Lipid Peroxidation / drug effects
  • Lipids / blood
  • Male
  • Molecular Docking Simulation
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Wistar
  • Seeds / chemistry
  • Seeds / metabolism
  • Superoxide Dismutase / metabolism
  • Terpenes / analysis
  • Terpenes / pharmacology
  • Terpenes / therapeutic use

Substances

  • Blood Glucose
  • Glycation End Products, Advanced
  • Lipids
  • Plant Extracts
  • Terpenes
  • Creatinine
  • Superoxide Dismutase
  • Glutathione

Grant support

The authors received no specific funding for this work.