Identification of CD4 and H-2K d-restricted cytotoxic T lymphocyte epitopes on the human herpesvirus 6B glycoprotein Q1 protein

Sci Rep. 2019 Mar 7;9(1):3911. doi: 10.1038/s41598-019-40372-5.

Abstract

The identification of Human herpesvirus 6B (HHV-6B) epitopes that are recognized by T-cells could contribute to the development of potential vaccines and immunotherapies. Here, we identified CD4+ and H-2Kd-restricted CD8+ T-cell epitopes on the glycoprotein Q1 of HHV-6B (BgQ1), which is a unique glycoprotein and essential for HHV-6B viral entry, by using in vivo electroporation with a plasmid DNA encoding BgQ1, overlapping peptides spanning the BgQ1 sequence, ELISPOT assay for quantification of gamma interferon (IFN-γ), and computer-based T-cell epitope prediction programs. The CD4+ and CD8+ T-cell epitopes identified in BALB/c mice in this study could be a good animal model system for use in the development of T-cell responses, inducing HHV-6B vaccines or immunotherapies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / immunology
  • Cell Line
  • Epitopes, T-Lymphocyte / immunology*
  • Glycoproteins / immunology
  • Herpesvirus 6, Human / immunology*
  • Humans
  • Interferon-gamma / immunology
  • Mice
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Antigens, Viral
  • Epitopes, T-Lymphocyte
  • Glycoproteins
  • Interferon-gamma