Gad1-promotor-driven GFP expression in non-GABAergic neurons of the nucleus endopiriformis in a transgenic mouse line

J Comp Neurol. 2019 Oct 1;527(14):2215-2232. doi: 10.1002/cne.24673. Epub 2019 Mar 20.


Transgenic animals have become a widely used model to identify and study specific cell types in whole organs. Promotor-driven reporter gene labeling of the cells under investigation has promoted experimental efficacy to a large degree. However, rigorous assessment of transgene expression specificity in these animal models is highly recommended to validate cellular identity and to isolate potentially mislabeled cell populations. Here, we report on one such mislabeled neuron population in a widely used transgenic mouse line in which GABAergic somatostatin-expressing interneurons (SOMpos INs) are labeled by eGFP (so-called GIN mouse, FVB-Tg(GadGFP)45704Swn/J). These neurons represent a subpopulation of all SOMpos INs. However, we report here on GFP labeling of non-GABAergic neurons in the nucleus endopiriformis of this mouse line.

Keywords: GABA; GAD67-promotor; RRID:AB_10000343; RRID:AB_10562708; RRID:AB_10563568; RRID:AB_10563770; RRID:AB_11180061; RRID:AB_2057032; RRID:AB_2255365; RRID:AB_2307443; RRID:AB_2338013; RRID:AB_2338078; RRID:AB_2338251; RRID:AB_2338680; RRID:AB_2338928; RRID:AB_258984; RRID:AB_310288; RRID:AB_477019; eGFP; interneurons; nucleus endopiriformis; off-target labeling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Claustrum / chemistry
  • Claustrum / metabolism*
  • GABAergic Neurons / chemistry
  • GABAergic Neurons / metabolism*
  • Gene Expression
  • Glutamate Decarboxylase / analysis
  • Glutamate Decarboxylase / biosynthesis*
  • Glutamate Decarboxylase / genetics
  • Green Fluorescent Proteins / analysis
  • Green Fluorescent Proteins / biosynthesis*
  • Green Fluorescent Proteins / genetics
  • Mice
  • Mice, Transgenic
  • Organ Culture Techniques
  • Piriform Cortex / chemistry
  • Piriform Cortex / metabolism*


  • Green Fluorescent Proteins
  • Glutamate Decarboxylase
  • glutamate decarboxylase 1