The cloning and characterization of a RAS gene from Schizosaccharomyces pombe

J Mol Evol. 1986;23(1):41-51. doi: 10.1007/BF02100997.


We have cloned and determined the complete nucleotide sequence of a RAS gene from the yeast Schizosaccharomyces pombe (SP-RAS). The putative RAS protein of 214 amino acids is encoded by two noncontiguous reading frames separated by an intron of 86 bp. The SP-RAS gene product shares extensive homology with the proteins of the Saccharomyces cerevisiae (SC), Dictyostelium, Drosophila, and human RAS genes in its N-terminal region but not in its C-terminal region. The extended C-terminal regions found in the SC-RAS genes have no counterpart in the SP-RAS gene. Thus the RAS genes of these two yeasts are structurally quite distinct. The SP-RAS sequence was expressed in vivo.

Publication types

  • Comparative Study

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Recombinant / analysis
  • Dictyostelium / genetics
  • Drosophila / genetics
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Humans
  • Neoplasm Proteins / genetics
  • Oncogene Protein p21(ras)
  • Oncogenes
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins p21(ras)
  • Saccharomyces cerevisiae / genetics
  • Saccharomycetales / genetics*
  • Schizosaccharomyces / genetics*
  • Sequence Homology, Nucleic Acid
  • ras Proteins*


  • DNA, Recombinant
  • Fungal Proteins
  • Neoplasm Proteins
  • Proto-Oncogene Proteins
  • ras protein, Dictyostelium
  • HRAS protein, human
  • Oncogene Protein p21(ras)
  • Proto-Oncogene Proteins p21(ras)
  • ras Proteins

Associated data

  • GENBANK/X03771