This study was conducted to evaluate the hepatoprotective effect of polysaccharides from Pleurotus ostreatus (POP) on carbon tetrachloride-induced acute liver injury (ALI) in rats. The hepatoprotective effect of POP against ALI was reflected by the decreased alanine transaminase and aspartate transaminase levels in blood, increased superoxide dismutase, catalase, and glutathione peroxidase levels, and decreased malondialdehyde levels in blood and liver. TUNEL and immunohistochemistry analyses showed that TUNEL apoptosis cell rate and Bax, cytochrome C, caspase-3, caspase-9, and apoptosis-inducing factor expression levels were obviously decreased, whereas Bcl-2 expression levels obviously increased after POP treatment. A total of 11 metabolites belonging to energy, amino acid, fatty acid, and gut bacteria metabolism were identified as biomarkers by using metabolite analysis of liver homogenate based on ultra-performance liquid chromatography-tandem mass spectrometry. POP exhibited hepatoprotective effects on CCl4-induced ALI, and the underlying mechanism is correlated with antioxidants that regulate metabolic pathway disorders and alleviate liver mitochondria apoptosis.
Keywords: Acute liver injury; Anti-apoptosis; Antioxidant; Metabolites; Pleurotus ostreatus; Polysaccharides.
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