Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I)

Stem Cell Res. 2019 Apr;36:101415. doi: 10.1016/j.scr.2019.101415. Epub 2019 Mar 2.

Abstract

The induced pluripotent stem cell (iPSC) lines ICGi008-A and ICGi008-B were generated from dermal fibroblasts using episomal vectors expressing pluripotency factors. Dermal fibroblasts were obtained from a 55 year old male Сaucasian familial Alzheimer's disease (AD) patient carrying heterozygous V717I mutation in the APP gene. The generated iPSC lines maintained the original APP genotype, expressed pluripotency markers, exhibited a normal karyotype and retained the ability to differentiate into cell types of the three germ layers. The iPSC lines will be useful for the study of the AD molecular and cellular mechanisms and drug screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / genetics*
  • Amyloid beta-Protein Precursor / genetics*
  • Cell Differentiation
  • Cell Line*
  • Fibroblasts
  • Genetic Markers
  • Heterozygote
  • Humans
  • Induced Pluripotent Stem Cells*
  • Karyotype
  • Male
  • Middle Aged
  • Skin

Substances

  • APP protein, human
  • Amyloid beta-Protein Precursor
  • Genetic Markers