A fluorometric assay for biotinidase

Anal Biochem. 1986 Apr;154(1):282-6. doi: 10.1016/0003-2697(86)90527-0.

Abstract

An assay for biotinidase using biocytin, the natural substrate, is described. The fluorometric procedure uses 1,2-diacetylbenzene which reacts selectively with lysine allowing its direct determination in mixture with biocytin. We have examined the applicability of the assay using human serum biotinidase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetophenones
  • Amidohydrolases / analysis*
  • Amidohydrolases / blood
  • Biotinidase
  • Colorimetry
  • Humans
  • Lysine / analogs & derivatives
  • Spectrometry, Fluorescence / methods*
  • Substrate Specificity

Substances

  • Acetophenones
  • 1,2-diacetylbenzene
  • Amidohydrolases
  • Biotinidase
  • biocytin
  • Lysine