Abstract
An assay for biotinidase using biocytin, the natural substrate, is described. The fluorometric procedure uses 1,2-diacetylbenzene which reacts selectively with lysine allowing its direct determination in mixture with biocytin. We have examined the applicability of the assay using human serum biotinidase.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetophenones
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Amidohydrolases / analysis*
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Amidohydrolases / blood
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Biotinidase
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Colorimetry
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Humans
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Lysine / analogs & derivatives
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Spectrometry, Fluorescence / methods*
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Substrate Specificity
Substances
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Acetophenones
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1,2-diacetylbenzene
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Amidohydrolases
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Biotinidase
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biocytin
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Lysine