Chorismate mutase isoenzymes from Sorghum bicolor: immunological characterization

Arch Biochem Biophys. 1986 May 1;246(2):617-21. doi: 10.1016/0003-9861(86)90317-6.


Highly purified fractions of chorismate mutase 1 and 2 from etiolated seedlings of Sorghum bicolor were used as the antigen for antibody production in BALB/c mice. Tests for antigen-antibody complex formation were made by immunodiffusion, immunoprecipitation, and enzyme-linked immunosorbent assay (ELISA). These tests indicated the presence of specific antibodies for each isoenzyme in their antisera. However, in the same tests, no cross-reaction was found between chorismate mutase 1 and 2 and their antisera. This indicates no immunological similarity between the two isoenzymes of chorismate mutase from sorghum.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibody Specificity
  • Chorismate Mutase / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Immunochemistry
  • Immunodiffusion
  • Isoenzymes / immunology*
  • Isomerases / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Plants / enzymology*


  • Isoenzymes
  • Isomerases
  • Chorismate Mutase