Accelerated infected wound healing by topical application of encapsulated Rosemary essential oil into nanostructured lipid carriers

Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):980-988. doi: 10.1080/21691401.2019.1582539.


The pathogenic bacteria delay wound healing due to their interaction in the wound area. This study is aimed to evaluate the efficiency of topical rosemary essential oil (REO) loaded into the nanostructured lipid carriers (NLCs) on in vitro antibacterial activity and in vivo infected wound healing process in the animal model. REO-NLCs morphology, size and in vitro antibacterial activity were done. Two circular full-thickness wound (each 6 mm) were made on the back of each mouse and each wound was infected with a solution containing 107 CFU Staphylococcus aureus and Pseudomonas aeruginosa. Animals were divided into four groups including control, Mupirocin® and two treated groups with a gel containing REO and REO-NLCs. For this purpose, tissue bacterial count, histological assessment, serum level of IL-3, IL-10, VEGF and SDF-1α were evaluated. REO-NLCs showed antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Pseudomonas aeruginosa. Moreover, REO-NLCs could reduce the rate of tissue bacterial colonization and wound size, while they increased the vascularization, fibroblast infiltration, re-epithelialization, collagen production, IL-3, IL-10, VEGF and SDF-1α serum levels. Our finding revealed the REO-NLCs have antibacterial properties and accelerated infected wound healing, and so that confirming their potential clinical uses for the treatment of infected wounds.

Keywords: Cytokines; Infected wound healing; Inflammation; Nanostructure lipid carrier; Rosemary essential oil.

MeSH terms

  • Administration, Topical
  • Animals
  • Anti-Bacterial Agents / administration & dosage*
  • Anti-Bacterial Agents / chemistry
  • Bacteria / drug effects
  • Bacteria / growth & development
  • Bacterial Load
  • Disease Models, Animal
  • Drug Carriers / chemistry*
  • Drug Delivery Systems
  • Inflammation Mediators / metabolism
  • Lipids / chemistry*
  • Mice
  • Microbial Sensitivity Tests
  • Nanostructures / administration & dosage*
  • Nanostructures / chemistry
  • Oils, Volatile / administration & dosage
  • Oils, Volatile / chemistry*
  • Treatment Outcome
  • Wound Healing / drug effects*
  • Wound Healing / physiology
  • Wound Infection / drug therapy*
  • Wound Infection / microbiology
  • Wound Infection / pathology


  • Anti-Bacterial Agents
  • Drug Carriers
  • Inflammation Mediators
  • Lipids
  • Oils, Volatile
  • rosemary oil