Characterization of polysaccharide from Scutellaria barbata and its antagonistic effect on the migration and invasion of HT-29 colorectal cancer cells induced by TGF-β1

Int J Biol Macromol. 2019 Jun 15;131:886-895. doi: 10.1016/j.ijbiomac.2019.03.053. Epub 2019 Mar 8.

Abstract

In this study, we purified a water-soluble polysaccharide, SBPW3, from the whole plant of Scutellaria barbata D. Don through ethanol precipitation, deproteinization, lyophilization, dialysis and separation using a DEAE cellulose column and a Superdex 200 gel filtration chromatography column. SBPW3 is a homogeneous polysaccharide with a molecular weight of 10.2 kDa and is composed of rhamnose (2.51%), arabinose (25.68%), xylose (10.94%), mannose (12.56%), glucose (20.59%) and galactose (27.72%). FT-IR spectrum analysis of the polysaccharide showed that SBPW3 contained a pyranose ring. The effects of SBPW3 on TGF-β1-induced epithelial-mesenchymal transition (EMT) were tested in colon cancer cells. These results suggested that SBPW3 significantly suppressed TGF-β1-induced migration and invasion. Additionally, SBPW3 reduced EMT by increasing the expression of epithelial markers and by decreasing the expression of mesenchymal markers by blocking the Smad2/3 signalling pathway in colon cancer cells. Furthermore, to explore the anti-metastatic effect of SBPW3, we established a mouse model of colon cancer metastasis and found that SBPW3 significantly inhibited the metastatic dissemination of the primary tumour to the liver. These findings provide us with a potential chemotherapeutic strategy for the treatment of human colorectal cancer.

Keywords: Anti-metastatic; Epithelial-mesenchymal transition; Polysaccharide; Scutellaria barbata D. Don.

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Cell Line, Tumor
  • Cell Movement / drug effects*
  • Disease Models, Animal
  • Epithelial-Mesenchymal Transition / drug effects
  • Gas Chromatography-Mass Spectrometry
  • HT29 Cells
  • Humans
  • Mice
  • Molecular Weight
  • Monosaccharides / chemistry
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Scutellaria / chemistry*
  • Signal Transduction / drug effects
  • Transforming Growth Factor beta1 / pharmacology*
  • Xenograft Model Antitumor Assays

Substances

  • Antineoplastic Agents, Phytogenic
  • Monosaccharides
  • Polysaccharides
  • Transforming Growth Factor beta1