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. 2019 Apr 1;218(4):1086-1088.
doi: 10.1083/jcb.201902041. Epub 2019 Mar 11.

No Chromosome Left Behind: The Importance of Metaphase Alignment for Mitotic Fidelity

Free PMC article

No Chromosome Left Behind: The Importance of Metaphase Alignment for Mitotic Fidelity

Bernardo Orr et al. J Cell Biol. .
Free PMC article


Chromosome alignment is a hallmark of mitosis in metazoans, but the physiological relevance of this orderly behavior has remained unclear. In this issue, Fonseca et al. (2019. J. Cell Biol. show that chromosome alignment ensures mitotic fidelity by promoting interchromosomal compaction during anaphase.


Figure 1.
Figure 1.
Chromosome alignment ensures anaphase synchrony to promote mitotic fidelity. Top panels (courtesy of Irina Matos and António Pereira) show illustrative chromokymographs of synchronous (A) and asynchronous (B) Drosophila melanogaster S2 cells undergoing anaphase (colors represent the tracking of individual kinetochores). Models illustrate the progression and fate for normal (A) and Kif18A-deficient (B) cells. Kif18A-deficient cells show impaired chromosome alignment and interchromosomal compaction during anaphase, leading to the formation of irregular daughter nuclei and/or micronuclei. Only a minor fraction of lagging chromosomes results in micronuclei, consistent with anaphase surveillance mechanisms (e.g., dependent on a midzone Aurora B phosphorylation gradient) that spatially control nuclear envelope reassembly. Cell proliferation in the presence of micronuclei is limited by p53, thereby ensuring genomic stability.

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