The present study was designed to elucidate the functional role of central amygdaloid nucleus (ACE) and mammillary body (MB) in the mediation of behavioral suppression using rat conflict punishment procedure. Lesion of ACE produced a significant and long-lasting increase in the punished responding during the experimental period. Rats with lesion of MB also showed a significant increase in the punished response 10-14 days after brain lesioning. These results demonstrated the important role of ACE and MB in the mediation of behavioral suppression such as conflict behavior.