Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1

Cell. 1986 Jun 6;45(5):667-74. doi: 10.1016/0092-8674(86)90781-6.

Abstract

Hemopoietin-1 (H-1) is known to act synergistically with CSF-1, a mononuclear phagocyte growth factor, to induce the development of primitive hemopoietic cells. To determine whether purified H-1 also acts on multipotent hemopoietic cells, its ability to act synergistically with interleukin-3 (IL-3) and erythropoietin (Epo) was tested in methyl cellulose cultures of murine bone marrow cells. In the presence of IL-3, H-1 increased the number of colonies formed by primitive, multipotent cells by approximately 30-fold. H-1 alone or H-1 plus Epo produced no colonies. Forty percent of the colonies induced by H-1 plus IL-3 contained cells that could be subcultured at least twice, whereas cells from colonies induced by IL-3 alone could not be similarly subcultured. Thus H-1 permits CSF-1 or IL-3 to act on cells more primitive than those acted on by either growth factor alone. The results indicate that H-1 acts on the most primitive hemopoietic cells yet shown to proliferate and differentiate in culture.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Colony-Forming Units Assay
  • Colony-Stimulating Factors / pharmacology
  • Drug Synergism
  • Erythropoietin / pharmacology
  • Female
  • Growth Substances / pharmacology*
  • Hematopoietic Cell Growth Factors
  • Hematopoietic Stem Cells / drug effects*
  • Interleukin-3
  • Lymphokines / pharmacology
  • Mice
  • Mice, Inbred C3H

Substances

  • Colony-Stimulating Factors
  • Growth Substances
  • Hematopoietic Cell Growth Factors
  • Interleukin-3
  • Lymphokines
  • Erythropoietin