By downregulating PBX3, miR-526b suppresses the epithelial-mesenchymal transition process in cervical cancer cells

Future Oncol. 2019 May;15(14):1577-1591. doi: 10.2217/fon-2018-0575. Epub 2019 Mar 12.

Abstract

Aim: Research on novel mutant genes may develop the treatment of cervical cancer (CC). The role of miRNA-526b in epithelial-mesenchymal transition (EMT) of CC was investigated. Methods: The role and the molecular mechanism of miRNA-526b in CC and its effect on EMT were analyzed in clinical specimens and oncology experiments. Results: miRNA-526b was proved to be decreased in CC and associated with malignant clinicopathological characters. The character of miRNA-526b in EMT was also inspected in CC cells and tumor models. miRNA-526b was found to be able to inhibit the EMT property of CC cells by directly targeting PBX3. Conclusion: miRNA-526b restoration may be deliberated as a new treatment strategy of CC.

Keywords: 3′-UTR; PBX3; cervical carcinoma; epithelial-to-mesenchymal transition; invasion; metastasis; miR-526b; miRNA-526; proliferation; tumor metastasis.

MeSH terms

  • 3' Untranslated Regions
  • Adult
  • Aged
  • Animals
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Disease Models, Animal
  • Epithelial-Mesenchymal Transition / genetics*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Homeodomain Proteins / genetics*
  • Humans
  • Mice
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Metastasis
  • Neoplasm Staging
  • Proto-Oncogene Proteins / genetics*
  • RNA Interference*
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / pathology*

Substances

  • 3' Untranslated Regions
  • Homeodomain Proteins
  • MIRN526 microRNA, human
  • MicroRNAs
  • Proto-Oncogene Proteins
  • proto-oncogene protein Pbx3