First fungemia case due to environmental yeast Wickerhamomyces myanmarensis: detection by multiplex qPCR and antifungal susceptibility

Future Microbiol. 2019 Mar;14(4):267-274. doi: 10.2217/fmb-2018-0253. Epub 2019 Mar 12.


Aim: Presenting the first clinical case of Wickerhamomyces myanmarensis.

Patients & methods: Yeast cells were isolated from blood and central venous catheter of a 5.5-year-old male subject. API 20C AUX, MALDI-TOF MS, ITS and LSU rDNA sequencing, and our qPCR assay were used for identification and the MIC values were determined by CLSI M27-A3.

Results: ITS and LSU rDNA sequencing identified both isolates as W. myanmarensis, while API 20C AUX and MALDI-TOF MS did not identify them correctly. Our qPCR specifically distinguished W. myanmarensis from W. anomalus. Isolate obtained from blood showed a higher MIC value for fluconazole, voriconazole and posaconazole.

Conclusion: Utilization of reliable identification tools might reveal the genuine spectrum of opportunistic yeast species.

Keywords: antifungal susceptibility testing; blood; central venous catheter; specific multiplex qPCR.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology*
  • Child
  • Drug Resistance, Fungal
  • Fluconazole / pharmacology
  • Fungemia / drug therapy
  • Fungemia / microbiology*
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Real-Time Polymerase Chain Reaction
  • Saccharomycetales / drug effects*
  • Saccharomycetales / genetics
  • Saccharomycetales / isolation & purification*
  • Triazoles / pharmacology
  • Voriconazole / pharmacology


  • Antifungal Agents
  • Triazoles
  • posaconazole
  • Fluconazole
  • Voriconazole