The Arabidopsis altered in stress response2 is Impaired in Resistance to Root and Leaf Necrotrophic Fungal Pathogens

Louise F Thatcher; Karam B Singh

doi:10.3390/plants8030060

The Arabidopsis altered in stress response2 is Impaired in Resistance to Root and Leaf Necrotrophic Fungal Pathogens

Plants (Basel). 2019 Mar 11;8(3):60. doi: 10.3390/plants8030060.

Authors

Louise F Thatcher¹, Karam B Singh^{2

3

4}

Affiliations

¹ CSIRO Agriculture and Food, Centre for Environment and Life Sciences, Wembley, Western Australia 6913, Australia. Louise.Thatcher@csiro.au.
² CSIRO Agriculture and Food, Centre for Environment and Life Sciences, Wembley, Western Australia 6913, Australia. Karam.Singh@csiro.au.
³ The Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia. Karam.Singh@csiro.au.
⁴ Centre for Crop and Disease Management, Department of Environment and Agriculture, Curtin University, Bentley, Western Australia 6102, Australia. Karam.Singh@csiro.au.

Abstract

The Arabidopsis thaliana Glutathione S-transferase Phi8 (GSTF8) gene is recognised as a marker for early defence and stress responses. To identify regulators of these responses, a forward genetic screen for Arabidopsis mutants with up-regulated GSTF8 promoter activity was conducted by screening a mutagenized population containing a GSTF8 promoter fragment fused to the luciferase reporter gene (GSTF8:LUC). We previously identified several enhanced stress response (esr) mutants from this screen that conferred constitutive GSTF8:LUC activity and increased resistance to several pathogens and/or insects pests. Here we identified a further mutant constitutively expressing GSTF8:LUC and termed altered in stress response2 (asr2). Unlike the esr mutants, asr2 was more susceptible to disease symptom development induced by two necrotrophic fungal pathogens; the root pathogen Fusarium oxysporum, and the leaf pathogen Alternaria brassicicola. The asr2 allele was mapped to a 2.1 Mbp region of chromosome 2 and narrowed to four candidate loci.

Keywords: At2g31320; At2g31400; At2g31990; At2g33170; At2g35170; At2g35740; At2g36420; At2g36835; At4g21670; At5g53060; CPL1; GSTF8; disease susceptibility; jasmonate; necrotroph.