Genetic polymorphisms in DNA repair genes and their association with cervical cancer

Br J Biomed Sci. 2019 Jul;76(3):117-121. doi: 10.1080/09674845.2019.1592884. Epub 2019 May 8.

Abstract

Background and objective: Carcinoma of cervix is the second most common cancer among women worldwide. The DNA repair network plays an important role in the maintenance of genetic stability, protection against DNA damage and carcinogenesis. Alterations in repair genes XRCC1, XRCC2 and XRCC3 and been reported in certain cancers. We hypothesised an association between XRCC1+399A/G, XRCC2+31467G/A and XRCC3+18067C/T polymorphisms and the risk of cervical cancer. Subjects and methods: This study included 525 subjects (265 controls and 260 cervical cancer cases). Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: Women with GA and AA genotypes of XRCC1+399A/G showed 2.4-3.8 fold higher risk of cervical cancer (P = 0.001). The +399A* allele was significantly linked with cervical cancer (P = 0.002). However, XRCC2+31479G/A and XRCC3+18067C/T polymorphisms did not show any statistically significant associations. Conclusion: The XRCC1+399A/G SNP is linked with cervical cancer. We suggest that this variant can be utilized as a prognostic marker for determination of cervical cancer susceptibility.

Keywords: Cervical cancer; PCR-RFLP; genetic polymorphism; repair genes.

MeSH terms

  • Adult
  • Aged
  • Alleles
  • DNA Repair / genetics*
  • DNA-Binding Proteins / genetics*
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease / genetics*
  • Genotype
  • Humans
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Uterine Cervical Neoplasms / genetics*
  • X-ray Repair Cross Complementing Protein 1 / genetics*

Substances

  • DNA-Binding Proteins
  • X-ray Repair Cross Complementing Protein 1
  • X-ray repair cross complementing protein 3
  • XRCC1 protein, human
  • XRCC2 protein, human