Lactate- And Acetate-Based Biohydrogen Production Through Dark Co-Fermentation of Tequila Vinasse and Nixtamalization Wastewater: Metabolic and Microbial Community Dynamics

Bioresour Technol. 2019 Jun;282:236-244. doi: 10.1016/j.biortech.2019.02.100. Epub 2019 Feb 23.


The aim of this work was to study the metabolic and microbial community dynamics during dark co-fermentation of 80% tequila vinasse and 20% nixtamalization wastewater (w/w). Batch co-fermentations were performed in a 3-L well-mixed reactor at 35 °C and pH 5.5. In correspondence to Illumina MiSeq sequencing and reactor monitoring, changes in metabolites and microbial communities were characterized by three main stages: (i) a first stage during which lactate and acetate producers dominated and consumed the major part of fermentable carbohydrates, (ii) a second stage in which lactate and acetate were consumed by emerging hydrogen-producing bacteria (HPB) in correlation with bioH2 (100 NmL/L-h or 1200 NmL/Lreactor) and butyrate production, and (iii) a third stage during which non-HPB outcompeted HPB after bioH2 production ceased. Altogether, the results of this study suggest that cooperative interactions between lactate producers and lactate- and acetate-consuming HPB could be attributed to lactate- and acetate-based cross-feeding interactions.

Keywords: Cross-feeding; Dark fermentation; Hydrogen-producing bacteria; Lactic acid bacteria; Microbial dynamics.

MeSH terms

  • Acetic Acid / metabolism*
  • Alcoholic Beverages*
  • Bioreactors / microbiology
  • Butyrates / metabolism
  • Fermentation*
  • Hydrogen / metabolism*
  • Lactic Acid / metabolism*
  • Microbiota*
  • Waste Water / chemistry*


  • Butyrates
  • Waste Water
  • Lactic Acid
  • Hydrogen
  • Acetic Acid