Collection of biochemical samples with brain-wide electrophysiological recordings from a freely moving rodent

J Pharmacol Sci. 2019 Apr;139(4):346-351. doi: 10.1016/j.jphs.2019.02.006. Epub 2019 Feb 27.


Bridging accumulating insights from microscopic and macroscopic studies in neuroscience research requires monitoring of neuronal population dynamics and quantifying specific molecules or genes from the brain of identical animals. To this end, by minimizing the size and weight of an electrode array, we developed a method that records local field potential signals of multiple brain regions from one side of the hemisphere in a freely moving rodent. At the same time, extracellular cerebrospinal fluid for biochemical assays or a small part of brain tissue samples for gene expression assays are collected from the other side of the hemisphere. This method allows ongoing stable recordings and sample collections for at least two months. The methodological concept is applicable to a wide range of biological reactions at various spatiotemporal scales, allowing us to integrate an idea of physiolomics into existing omics analyses, leading to a new combination of multi-omics approaches.

Keywords: Brain; Genes; Local field potential; Microdialysis; Neuromodulator.

MeSH terms

  • Animals
  • Brain / metabolism
  • Brain / physiology*
  • Electrophysiological Phenomena*
  • Gene Expression
  • Male
  • Microdialysis
  • Motor Activity*
  • Neurosciences / methods*
  • Neurotransmitter Agents / metabolism
  • Rats, Wistar / physiology*


  • Neurotransmitter Agents