DamID as a versatile tool for understanding gene regulation

Development. 2019 Mar 15;146(6):dev173666. doi: 10.1242/dev.173666.


The interaction of proteins and RNA with chromatin underlies the regulation of gene expression. The ability to profile easily these interactions is fundamental for understanding chromatin biology in vivo DNA adenine methyltransferase identification (DamID) profiles genome-wide protein-DNA interactions without antibodies, fixation or protein pull-downs. Recently, DamID has been adapted for applications beyond simple assaying of protein-DNA interactions, such as for studying RNA-chromatin interactions, chromatin accessibility and long-range chromosome interactions. Here, we provide an overview of DamID and introduce improvements to the technology, discuss their applications and compare alternative methodologies.

Keywords: Chromatin; DamID; Gene regulation; Genomics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Binding Sites / genetics
  • Chromatin / metabolism*
  • DNA Methylation*
  • Drosophila melanogaster / metabolism
  • Gene Expression Regulation
  • Genetic Techniques*
  • Genomics
  • Humans
  • Mice
  • Protein Binding
  • Ribosomes / metabolism
  • Sequence Analysis, DNA
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism*


  • Chromatin
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)