On-site quantitative Hg2+ measurements based on selective and sensitive fluorescence biosensor and miniaturized smartphone fluorescence microscope

Abstract

Mercury is a bio-accumulative and toxic pollutant causing severe damages to human health and environment. Since Hg²⁺ is the most stable form of mercury, selective and sensitive Hg²⁺ detection is required. Though classical approaches can realize accurate Hg²⁺ detection, the complicated instruments and the time-consuming operations inevitably limit their on-site applications. Here, we design a smart Hg²⁺ detection approach using the fluorescence biosensor, the smartphone fluorescence microscope and the smartphone application for Hg²⁺ on-site detection. Based on the thymine-Hg²⁺-thymine coordination chemistry, a selective and sensitive fluorescence biosensor is designed for capturing Hg²⁺ in aqueous solution; besides, a miniaturized smartphone fluorescence microscope for fluorescence signal collection and an image processing application for quantitative Hg²⁺ measurements are constructed. A highly specific detection of Hg²⁺ with a linear relation between 1 nM and 1 μM with a limit of detection of 1 nM is obtained using the smart Hg²⁺ detection approach. Considering it can realize selective and sensitive quantitative Hg²⁺ measurements in high precision with simple operations and cost-effective system, it is believed the proposed smart Hg²⁺ detection approach owns great potentials in Hg²⁺ detection for routine uses at home and in the field.

Keywords: On-site detection; Quantitative Hg(2+) measurements; Smart Hg(2+) detection method; Smartphone fluorescence microscope; Thymine-Hg(2+)-thymine coordination chemistry.