Growth of Clostridium difficile and production of toxins A and B in complex and defined media

J Med Microbiol. 1986 Jun;21(4):293-7. doi: 10.1099/00222615-21-4-293.


The ability of several strains of Clostridium difficile to grow and to produce toxins A and B in complex and defined culture media has been studied with special reference to the amino-acid composition of the medium. The production of these toxins varied with the strain used and with the composition of the growth medium. Toxin A production was not inextricably linked to production of toxin B since conditions were found in which only one or other toxin was produced.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / physiology
  • Bacterial Proteins*
  • Bacterial Toxins / biosynthesis*
  • Clostridium / drug effects
  • Clostridium / growth & development*
  • Clostridium / metabolism
  • Culture Media / pharmacology
  • Enterotoxins*


  • Amino Acids
  • Bacterial Proteins
  • Bacterial Toxins
  • Culture Media
  • Enterotoxins
  • tcdA protein, Clostridium difficile
  • toxB protein, Clostridium difficile