Efficacy of an orally administered anti-diarrheal probiotic paste (Pro-Kolin Advanced) in dogs with acute diarrhea: A randomized, placebo-controlled, double-blinded clinical study

J Vet Intern Med. 2019 May;33(3):1286-1294. doi: 10.1111/jvim.15481. Epub 2019 Mar 18.


Background: Acute diarrhea is a common clinical presentation of dogs. The effect of specific anti-diarrheal probiotic pastes (ADPPs) in the management of acute, uncomplicated diarrhea in dogs is unknown.

Hypothesis: Administration of an ADPP containing Enterococcus faecium 4b1707 will improve the clinical outcome of acute, uncomplicated diarrhea in dogs compared to placebo.

Animals: One hundred forty-eight client-owned dogs with acute diarrhea as the main clinical sign.

Methods: Double-blinded, placebo-controlled, randomized, blocked, multicenter clinical field study conducted at 14 primary care veterinary practices in the United Kingdom and Ireland.

Results: The ADPP was associated with better clinical outcome compared to placebo in dogs with acute, uncomplicated diarrhea. Dogs in the ADPP group had a significantly shorter duration of diarrhea (ADPP: median, 32 hours; 95% confidence interval [CI], 2-118; n = 51; Placebo: median, 47 hours; 95% CI, 4-167; n = 58; P = .008) and the rate of resolution of diarrhea was 1.60 times faster in the ADPP group than in the Placebo group (ratio, 1.60; 95% CI, 1.08-2.44; P = .02). Fewer dogs required additional medical intervention (AMI) for non-improvement or worsening in the ADPP group compared to the Placebo group (3.5% of dogs and 14.8% of dogs, respectively), with a relative risk of 0.88 (P = .04; AMI, ADPP, 3.5%, 2/57 dogs; Placebo, 14.8%, 9/61 dogs; relative risk, 0.88; 95% CI, 0.77-0.99).

Conclusion and clinical importance: The ADPP may accelerate resolution of acute diarrhea in dogs and decrease the requirement for AMI.

Keywords: Enterococcus faecium; Pro-Kolin Advanced; kaolin; montmorillonite; pectin; prebiotic.

Publication types

  • Clinical Trial, Veterinary
  • Multicenter Study

MeSH terms

  • Animals
  • Diarrhea / drug therapy
  • Diarrhea / veterinary*
  • Dog Diseases / drug therapy*
  • Dogs
  • Double-Blind Method
  • Enterococcus faecium*
  • Female
  • Ireland
  • Male
  • Probiotics / administration & dosage
  • Probiotics / therapeutic use*
  • Random Allocation
  • United Kingdom