Rhodopseudomonas palustris wastewater treatment: Cyhalofop-butyl removal, biochemicals production and mathematical model establishment

Pan Wu; Zhaobo Chen; Ying Zhang; Yanling Wang; Feifei Zhu; Bo Cao; Yuan Wu; Ning Li

doi:10.1016/j.biortech.2018.11.087

Rhodopseudomonas palustris wastewater treatment: Cyhalofop-butyl removal, biochemicals production and mathematical model establishment

Bioresour Technol. 2019 Jun:282:390-397. doi: 10.1016/j.biortech.2018.11.087. Epub 2019 Jan 19.

Authors

Pan Wu¹, Zhaobo Chen¹, Ying Zhang², Yanling Wang³, Feifei Zhu⁴, Bo Cao⁵, Yuan Wu¹, Ning Li¹

Affiliations

¹ School of Environment and Resources, Dalian Minzu University, Dalian 116600, China; School of Resources and Environment, Northeast Agricultural University, ChinSchool of Environment and Resources, Harbin 150030, China.
² School of Environment and Resources, Dalian Minzu University, Dalian 116600, China; School of Resources and Environment, Northeast Agricultural University, ChinSchool of Environment and Resources, Harbin 150030, China. Electronic address: mowentao05g@163.com.
³ Department of Anesthesiology, the Third Affiliated Hospital of SunYat-Sen University, Guangzhou 510630, China.
⁴ Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China.
⁵ School of Environment and Resources, Dalian Minzu University, Dalian 116600, China.

PMID: 30884459
DOI: 10.1016/j.biortech.2018.11.087

Abstract

Simultaneous (SPW and cyhalofop-butyl) wastewater treatment and the production of biochemicals by Rhodopseudomonas palustris (R. palustris) was investigated with supplementation of soybean processing wastewater (SPW). Compared to control group, cyhalofop-butyl was removed and single cell protein, carotenoid, bacteriochlorophyll productions were enhanced with the supplementation of SPW. Cyhalofop-butyl removal reached 100% after 5 days under 4000 mg/L COD group. Cyhalofop-butyl induced chbH gene expression to synthesize cyhalofop-butyl-hydrolyzing carboxylesterase through activating MAPKKKs, MAPKKs, MAPKs genes in MAPK signal transduction pathway. The induction process took one day for R. palustris. However, lack of organics in original wastewater did not maintain R. palustris growth for over one day. The supplementation of SPW provided sufficient carbon source. This new method resulted in the mixed wastewater treatment and improvement of biochemicals simultaneously, as well as the realization of reutilization of R. palustris. High-order non-linear mathematical model of the relationship between R_chb, X_c, and X_t was established.

Keywords: Cyhalofop-butyl wastewater; Cyhalofop-butyl-hydrolyzing carboxylesterase; Mathematical model; R. palustris; chbH gene.

MeSH terms

Biochemical Phenomena
Butanes / metabolism*
Models, Theoretical
Nitriles / metabolism*
Rhodopseudomonas / metabolism*
Waste Disposal, Fluid / methods*
Wastewater / chemistry*

Substances

Butanes
Nitriles
Waste Water
cyhalofop-butyl