Serum microRNAs and chronic periodontitis: A case-control study

Arch Oral Biol. 2019 May:101:57-63. doi: 10.1016/j.archoralbio.2019.03.009. Epub 2019 Mar 13.


Objective: An association is present between periodontitis and rates of expression of certain microRNAs (miRNAs) in periodontal tissue. However, the association between periodontitis and miRNA levels in human serum is unknown. We performed a case-control study in patients with chronic periodontitis to investigate serum miRNA levels.

Design: We enrolled 30 healthy patients without periodontitis and 30 patients with chronic periodontitis. Participants underwent clinical examination, case selection, and a blood draw from the antecubital vein. Serum miRNA profiles were compared in samples from participants with and without chronic periodontitis using microarray and real-time PCR.

Results: Microarray demonstrated seven miRNAs that were expressed <1/1.5 or >1.5 in the control group compared to the periodontitis group (p < 0.05). Real-time PCR showed that hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p were higher in the periodontitis group than the control group (p < 0.05).

Conclusion: Hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p are candidate serum biomarkers for chronic periodontitis.

Keywords: Biomarkers; Case-control study; Periodontitis; microRNAs; serum.

MeSH terms

  • Biomarkers / blood
  • Case-Control Studies
  • Chronic Periodontitis / blood*
  • Gene Expression Profiling
  • Humans
  • MicroRNAs / blood*
  • Real-Time Polymerase Chain Reaction


  • Biomarkers
  • MIRN21 microRNA, human
  • MIRN501 microRNA, human
  • MIRN664 microRNA, human
  • MicroRNAs