Biological synergy of greener gold nanoparticles by using Coleus aromaticus leaf extract

P Boomi; R M Ganesan; G Poorani; H Gurumallesh Prabu; S Ravikumar; J Jeyakanthan

doi:10.1016/j.msec.2019.01.105

Biological synergy of greener gold nanoparticles by using Coleus aromaticus leaf extract

Mater Sci Eng C Mater Biol Appl. 2019 Jun:99:202-210. doi: 10.1016/j.msec.2019.01.105. Epub 2019 Jan 25.

Authors

P Boomi¹, R M Ganesan², G Poorani³, H Gurumallesh Prabu⁴, S Ravikumar⁵, J Jeyakanthan⁶

Affiliations

¹ Department of Bioinformatics, Alagappa University, Karaikudi 630003, Tamil Nadu, India. Electronic address: pboomi1983@gmail.com.
² Department of Chemistry, Arumugam Seethaiyammal Arts and Science College, Tiruppattur, Tamil Nadu, India.
³ Kumaraguru College of Technology, Coimbatore 641049, Tamil Nadu, India.
⁴ Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
⁵ Department of Biomedical Sciences, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
⁶ Department of Bioinformatics, Alagappa University, Karaikudi 630003, Tamil Nadu, India.

PMID: 30889692
DOI: 10.1016/j.msec.2019.01.105

Abstract

Greener nanotechnology plays an important role in developing alternative and effective treatment strategies for various diseases. Biological synthesis of metal nanoparticles (MNPs) has known to possess suitable alternatives than the existing chemical methods. Greener synthesis of MNPs by using plant extracts has become an emerging field due to their safe, eco-friendly and non-toxic nature that are suitable for synergistic biological activities. Hence, the greener method is chosen by the present study. In the present study, the greener synthesis of gold nanoparticles (AuNPs) was successfully done by using Coleus aromaticus leaf extract at three different temperatures (30 °C, 60 °C and 100 °C). The formation of AuNPs was initially monitored by visual observation and then characterized with the help of diverse techniques like UV-Vis spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM), High Resolution-Transmission Electron Microscopy (HR-TEM) and dynamic light scattering (DLS). Surface plasmon resonance (SPR) peak and crystalline nature of AuNPs were obtained by UV-Vis and XRD spectroscopies respectively. FT-IR analysis shows the different characteristic functional groups in turn responsible for the bio-reduction of gold ions by using leaf extract. The formations of different nano-sized AuNPs with their different morphologies were observed by SEM and HR-TEM analyses. Surface charge and stability of the AuNPs were measured by zeta potential and DLS respectively. The synthesized AuNPs coated with cotton fabric was analyzed by UV-Diffuse Reflectance Spectroscopy (UV-DRS), which revealed excellent UV protection against UV radiation. The AuNPs coated cotton fabric exhibited remarkable antibacterial sensitivity against Staphylococcus epidermidis and Escherichia coli. Further, the synthesized AuNPs showed significant cytotoxicity against human liver cancer (HepG2) cell line. The findings of this study revealed that, AuNPs synthesized using Coleus aromaticus leaf extract could be an alternative, safe, and effective source of UV protection, antibacterial and anticancer agents.

Keywords: Antibacterial activity; Au nanoparticles; Coleus aromaticus; Cytotoxicity; UV protection.

MeSH terms

Anti-Bacterial Agents / pharmacology
Cell Death / drug effects
Coleus / chemistry*
Cotton Fiber
Dynamic Light Scattering
Gold / chemistry*
Green Chemistry Technology / methods*
Hep G2 Cells
Humans
Inhibitory Concentration 50
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Microbial Sensitivity Tests
Plant Extracts / chemistry*
Plant Leaves / chemistry*
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Anti-Bacterial Agents
Plant Extracts
Gold