Identification of new DNA-associated proteins from Waddlia chondrophila

Sci Rep. 2019 Mar 20;9(1):4885. doi: 10.1038/s41598-019-40732-1.


Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ66), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics*
  • Chlamydiaceae Infections / microbiology
  • Chlamydiales / genetics*
  • Chlorocebus aethiops
  • DNA-Binding Proteins / genetics*
  • HEK293 Cells
  • Humans
  • Vero Cells


  • Bacterial Proteins
  • DNA-Binding Proteins

Supplementary concepts

  • Waddlia chondrophila