Application of Drosophila Model Toward Understanding the Molecular Basis of Fragile X Syndrome

Methods Mol Biol. 2019;1942:141-153. doi: 10.1007/978-1-4939-9080-1_12.

Abstract

Drosophila melanogaster is an ideal model to study Fragile X syndrome (FXS) as it presents us with a toolbox to identify genetic modifiers and to investigate the molecular mechanisms of FXS. Here we describe some of the methods that have been used to study FXS, ranging from reverse genetic screening using the GAL4-UAS system, to mushroom body staining and courtship behavioral assays to examine the learning and memory deficits associated with FXS.

Keywords: Courtship behavioral assay; Drosophila genetic screen; Fragile X syndrome; Mushroom body staining.

MeSH terms

  • Animals
  • Behavior, Animal
  • Courtship
  • Disease Models, Animal*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism*
  • Eye / physiopathology
  • Female
  • Fragile X Mental Retardation Protein / genetics
  • Fragile X Mental Retardation Protein / metabolism*
  • Fragile X Syndrome / physiopathology*
  • Learning Disabilities / physiopathology*
  • Male
  • Memory Disorders / physiopathology*
  • Mushroom Bodies / physiopathology

Substances

  • Drosophila Proteins
  • Fragile X Mental Retardation Protein