Simulation of in situ biodegradation of 1,4-dioxane under metabolic and cometabolic conditions

Abstract

Bioaugmentation is an option for aerobic remediation of groundwater contaminated with 1,4-dioxane. One approach uses microbes that cometabolize 1,4-dioxane following growth on a primary substrate (e.g., propane), whereas another uses microbes (e.g., Pseudonocardia dioxivorans CB1190) capable of using 1,4-dioxane as a sole substrate. The relative merits of these approaches are difficult to distinguish based on field data alone, and theoretical analyses of these processes have yet to be published. The objective of this study was to compare these remediation options using a transport model that incorporates advection, dispersion and biodegradation reactions described by multi-substrate Monod kinetics and co-inhibition effects. The transport model was coupled to an approximate steady-state air sparging simulation used to estimate gas (propane and oxygen) distribution at the field scale. The model was calibrated with field data for 1,4-dioxane and propane concentrations from a previously reported pilot study. The two remediation approaches were evaluated under different conditions that vary the initial concentration of 1,4-dioxane and the loading rates of oxygen, propane, and biomass. The metrics used to evaluate the remediation success were the time to reach an average 1,4-dioxane concentration of 1 μg L^-1 and the percent of 1,4-dioxane biodegraded after 10 years of simulation. Results indicate that the initial concentration of 1,4-dioxane strongly influences which remediation approach is more effective. When initial concentrations were <10 mg L^-1, propane-driven cometabolism led to faster remediation; whereas metabolic biodegradation was faster when initial concentrations were 10 mg L^-1 or higher. Below 0.25 mg L^-1, the viability of metabolic biodegradation improved, although cometabolism by propanotrophs still required less time to reach 1 μg L^-1. Biomass injection rates had a strong effect on the rate of metabolism but not cometabolism because continuous input of primary substrate supported growth of propanotrophs. The performance of both cultures was negatively affected by a decrease in oxygen injection rate. The endogenous decay coefficient and the dispersion rate for biomass had a significant impact on cometabolic and metabolic biodegradation of 1,4-dioxane. The maximum specific rate for cometabolism of 1,4-dioxane, the dispersion rate for 1,4-dioxane, and effective porosity also had significant effects on the time to achieve remediation with propanotrophs.

Keywords: 1,4-Dioxane; Bioaugmentation; Biodegradation; Biosparging; Contaminant transport model.