Effect of fluoride mouthrinse and fluoride concentration on bonding of a one-step self-etch adhesive to bovine root dentin

J Oral Sci. 2019;61(1):125-132. doi: 10.2334/josnusd.17-0466.


This study investigated the effect of fluoride mouthrinses on bonding of a one-step self-etch adhesive to bovine root dentin. Application of a NaF solution (0, 450, 900, or 9,000 ppm) to bovine root dentin surfaces was performed for 30 s (immediate) or before bonding of an all-in-one adhesive. Microtensile bond strength (µTBS) testing and scanning electron microscopic (SEM) observation of the acid-base resistance zone (ABRZ) were performed. µTBS values and calculated ABRZ areas were analyzed by using two-way ANOVA and the t-test with Bonferroni correction. The significance level was set at P = 0.05 (n = 30). Fracture mode was analyzed with the Mann-Whitney U test with Bonferroni correction. The significance level set at P = 0.00178 (n = 30). µTBS did not significantly differ between the control, 450 ppm F, and 900 ppm F specimens (P > 0.05) but was significantly lower in 9,000 ppm F specimens (P < 0.05). SEM showed a significant increase in acid resistance at the adhesive-dentin interface, including the ABRZ, after fluoride application.

Keywords: ABRZ; bovine root dentin; fluoride mouthrinse; one-step self-etching adhesive; µTBS.

MeSH terms

  • Acid Etching, Dental*
  • Animals
  • Cattle
  • Dental Cements / chemistry*
  • Dental Stress Analysis
  • Dentin / chemistry*
  • Fluorides / administration & dosage*
  • Microscopy, Electron, Scanning
  • Mouthwashes*
  • Surface Properties
  • Tooth Fractures
  • Tooth Root*


  • Dental Cements
  • Mouthwashes
  • Fluorides