Ventral tegmental area astrocytes orchestrate avoidance and approach behavior

Nat Commun. 2019 Mar 29;10(1):1455. doi: 10.1038/s41467-019-09131-y.

Abstract

The ventral tegmental area (VTA) is a heterogeneous midbrain structure, containing neurons and astrocytes, that coordinates behaviors by integrating activity from numerous afferents. Within neuron-astrocyte networks, astrocytes control signals from distinct afferents in a circuit-specific manner, but whether this capacity scales up to drive motivated behavior has been undetermined. Using genetic and optical dissection strategies we report that VTA astrocytes tune glutamatergic signaling selectively on local inhibitory neurons to drive a functional circuit for learned avoidance. In this circuit, astrocytes facilitate excitation of VTA GABA neurons to increase inhibition of dopamine neurons, eliciting real-time and learned avoidance behavior that is sufficient to impede expression of preference for reward. Loss of one glutamate transporter (GLT-1) from VTA astrocytes selectively blocks these avoidance behaviors and spares preference for reward. Thus, VTA astrocytes selectively regulate excitation of local GABA neurons to drive a distinct avoidance circuit that opposes approach behavior.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Transport System X-AG / metabolism
  • Animals
  • Astrocytes / physiology*
  • Avoidance Learning / physiology*
  • Choice Behavior / physiology*
  • Dopaminergic Neurons / metabolism
  • Female
  • GABAergic Neurons / physiology
  • Glutamic Acid / metabolism
  • Male
  • Mice, Inbred C57BL
  • Models, Biological
  • Neural Inhibition
  • Ventral Tegmental Area / cytology*

Substances

  • Amino Acid Transport System X-AG
  • Glutamic Acid