Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation

Diagn Microbiol Infect Dis. 2019 Aug;94(4):349-354. doi: 10.1016/j.diagmicrobio.2019.02.006. Epub 2019 Feb 10.

Abstract

The goal of the study was to adapt our Middle East respiratory syndrome coronavirus (MERS-CoV) lab-developed test (LDT) to 3 "Sample to Result" (S2R) systems: BD MAX (BD), ELITe InGenius (ELITechGroup), and ARIES (Luminex). The BD MAX and InGenius system allowed use of lab-developed primers and TaqMan probes, while ARIES required conversion to MultiCode primers for melting curve analysis. Each device required ≤1 day of training and assay optimization. No discordant results were noted after analysis of 32 External Quality Control (EQC) samples. On a 10-fold dilution series of a MERS-CoV-positive EQC sample, InGenius obtained the highest detection rate. Laboratory technicians rated the ARIES as the user-friendliest. It also required the least hands-on time. BD MAX had the lowest turnaround time and highest throughput. While each device had distinguishing system properties with associated (dis)advantages, the 3 S2R systems were comparable in terms of assay development and validation.

Keywords: ARIES; BD MAX; ELITe InGenius; Middle East respiratory syndrome coronavirus; Sample to result systems.

Publication types

  • Validation Study

MeSH terms

  • Clinical Laboratory Techniques
  • Coronavirus Infections / diagnosis
  • DNA Primers
  • Middle East Respiratory Syndrome Coronavirus / genetics
  • Middle East Respiratory Syndrome Coronavirus / isolation & purification*
  • RNA, Viral / genetics
  • Real-Time Polymerase Chain Reaction / methods*
  • Real-Time Polymerase Chain Reaction / standards*

Substances

  • DNA Primers
  • RNA, Viral