Regulatory roles of PGE2 in LPS-induced tissue damage in bovine endometrial explants

Abstract

Bovine endometritis is the most common uterine disease following parturition. The role of prostaglandin E₂ (PGE₂) in regulating normal physiological function in the bovine endometrium has been clearly established. Although PGE₂ accumulation is observed in multiple inflammatory diseases, such as endometritis, its association with pathogen-induced inflammatory damage in the endometrium is unclear. To clarify the role of PGE₂ in lipopolysaccharide (LPS)-induced endometritis in cultured bovine endometrial explants, the levels of PGE₂ secretion, prostaglandin synthetases, pro-inflammatory factors, and damage-associated molecular patterns (DAMPs) were evaluated in the present study. Significant PGE₂ accumulation in response to LPS stimulation, up-regulation of prostaglandin-endoperoxide synthase-2 (PTGS-2), microsomal prostaglandin E synthase-1 (mPGES-1), pro-inflammatory factors including interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and induced nitric oxide synthase (iNOS)/nitric oxide (NO) and DAMPs including hyaluronan binding protein 1 (HABP1) and high mobility group box-1 (HMGB1), were observed compared to the control group. LPS induced distinct damage in the bovine endometrium, characterized by morphological changes and increases in HABP1 and HMGB1 expression. PTGS-2 inhibitors CAY10404 and NS398 effectively decreased the secretion of PGE₂ and the expression of prostaglandin synthetases, pro-inflammatory factors and DAMPs, and alleviated LPS-induced tissue damage. These results indicate that PGE₂ accumulates via PTGS-2 and mPGES-1 and induces tissue damage by upregulating pro-inflammatory factors and DAMPs in LPS-treated bovine endometrial explants. These findings provide a basis for the effect of PGE₂ on LPS-treated bovine endometrium, and suggest a potential target for curing endometritis.

Keywords: Cytokines; Damage-associated molecular patterns; Endometrium; Lipopolysaccharide; Prostaglandins.