Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

PLoS One. 2019 Apr 1;14(4):e0210374. doi: 10.1371/journal.pone.0210374. eCollection 2019.

Abstract

Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computational Biology
  • DNA / chemistry*
  • DNA / genetics
  • DNA Fragmentation*
  • Deoxyribonuclease I / chemistry
  • Gene Library
  • High-Throughput Nucleotide Sequencing / methods*
  • Polymerization*
  • Sequence Analysis, DNA / methods*

Substances

  • DNA
  • Deoxyribonuclease I

Grant support

The work was supported by the Ministry of Education and Science of Russian Federation, grant 14.579.21.0012 (ID # RFMEFI57914X0012) and by the grant 0112-2016-0006 from the Russian State Budget. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Syntol JSC provided support in the form of salary for Y.A.M. and reagents for NGS-analysis, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of this author are articulated in the ‘author contributions’ section. Evrogen JSC provided equipment for NGS-analysis and provided support in the form of salary for author K.A.B., but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section. Bioron GmbH provided enzymes and reagents (including SD DNA polymerase), but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.